› 1stc

X-ray diffraction
2.3Å resolution

CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT IN COMPLEX WITH STAUROSPORINE

Released:
DOI: 10.2210/pdb1stc/pdb
PDB entry 1stc coloured by chain, front view.
PDB entry 1stc coloured by chain, side view.
PDB entry 1stc coloured by chain, top view.
Source organisms:
Primary publication:
Staurosporine-induced conformational changes of cAMP-dependent protein kinase catalytic subunit explain inhibitory potential.
Prade L, Engh RA, Girod A, Kinzel V, Huber R, Bossemeyer D
Structure 5 1627-37 (1997)
PMID: 9438863

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132591 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.79 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00517 (Residues: 2-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Oryctolagus cuniculus
Expression system: Not provided
UniProt:
  • Canonical: P61926 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

1 bound ligand:
Ligand STU 1 x STU
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 68.99Å b: 72.82Å c: 76.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.33
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Magic bullets for protein kinases.
Bishop et al. (2001)
14 more

14 mentions without citation

Solution NMR Spectroscopy for the Study of Enzyme Allostery.
Lisi et al. (2016)
13 more