› 1svo

X-ray diffraction
2.6Å resolution

Structure of SV40 large T antigen helicase domain

Released:
DOI: 10.2210/pdb1svo/pdb
PDB entry 1svo coloured by chain, front view.
PDB entry 1svo coloured by chain, side view.
PDB entry 1svo coloured by chain, top view.
Source organism: Macaca mulatta polyomavirus 1
Primary publication:
Mechanisms of conformational change for a replicative hexameric helicase of SV40 large tumor antigen.
Gai D, Zhao R, Li D, Finkielstein CV, Chen XS
Cell 119 47-60 (2004)
PMID: 15454080

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 43.54 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03070 (Residues: 251-627; Coverage: 53%)
Sequence domains: Polyomavirus large T antigen C-terminus
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P321
Unit cell:
a: 120.444Å b: 120.444Å c: 131.767Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.271
Expression system: Escherichia coli

Quick links

Citations

52 review citations

Molecular chaperones and protein quality control.
Bukau et al. (2006)
51 more

10 mentions without citation

Understanding helicases as a means of virus control.
Frick et al. (2006)
9 more