› 1swb

X-ray diffraction
1.85Å resolution

APO-CORE-STREPTAVIDIN AT PH 7.5

Released:
DOI: 10.2210/pdb1swb/pdb
PDB entry 1swb coloured by chain, front view.
PDB entry 1swb coloured by chain, side view.
PDB entry 1swb coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Structural studies of the streptavidin binding loop.
Freitag S, Le Trong I, Klumb L, Stayton PS, Stenkamp RE
Protein Sci 6 1157-66 (1997)
PMID: 9194176

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 58.9Å b: 88.2Å c: 47.4Å
α: 90° β: 98.7° γ: 90°
R-values:
R R work R free
0.174 not available 0.253
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Attenuated total reflection infrared spectroscopy of proteins and lipids in biological membranes.
Goormaghtigh et al. (1999)
5 more

17 mentions without citation

Top-Down Proteomics: Ready for Prime Time?
Chen et al. (2018)
16 more