› 1sxy

X-ray diffraction
1.07Å resolution

1.07 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus

Released:
DOI: 10.2210/pdb1sxy/pdb
PDB entry 1sxy coloured by chain, front view.
PDB entry 1sxy coloured by chain, side view.
PDB entry 1sxy coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4.
Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR
Biochemistry 43 6679-90 (2004)
PMID: 15157102

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand NH4 1 x NH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 70.142Å b: 42.776Å c: 52.979Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.151 0.15 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Iron transitions during activation of allosteric heme proteins in cell signaling.
Négrerie M. (2019)
38 other articles

1 mention without citation

NMR studies of the dynamics of nitrophorin 2 bound to nitric oxide.
Muthu et al. (2013)