› 1sza

X-ray diffraction
2.2Å resolution

The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model

Released:
DOI: 10.2210/pdb1sza/pdb
PDB entry 1sza coloured by chain, front view.
PDB entry 1sza coloured by chain, side view.
PDB entry 1sza coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.
Meinhart A, Cramer P
Nature 430 223-6 (2004)
PMID: 15241417

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153822 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein PCF11 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 144 amino acids
Theoretical weight: 16.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39081 (Residues: 1-140; Coverage: 22%)
Gene names: PCF11, YD9934.13C, YDR228C
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
CTD-peptide Chain: Z
Molecule details ›
Chain: Z
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 57.62Å b: 67Å c: 135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

47 review citations

Progression through the RNA polymerase II CTD cycle.
Buratowski S. (2009)
46 more

13 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
12 more