› 1szo

X-ray diffraction
1.9Å resolution

Crystal Structure Analysis of the 6-Oxo Camphor Hydrolase His122Ala Mutant Bound to Its Natural Product (2S,4S)-alpha-Campholinic Acid

Released:
DOI: 10.2210/pdb1szo/pdb
PDB entry 1szo coloured by chain, front view.
PDB entry 1szo coloured by chain, side view.
PDB entry 1szo coloured by chain, top view.
Source organism: Rhodococcus sp. NCIMB 9784
Primary publication:
Structure of 6-oxo camphor hydrolase H122A mutant bound to its natural product, (2S,4S)-alpha-campholinic acid: mutant structure suggests an atypical mode of transition state binding for a crotonase homolog.
Leonard PM, Grogan G
J Biol Chem 279 31312-7 (2004)
PMID: 15138275

Function and Biology Details

Reaction catalysed: 
Bornane-2,6-dione + H(2)O = ((1S)-4-hydroxy-2,2,3-trimethylcyclopent-3-enyl)acetate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-188133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-oxocamphor hydrolase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 257 amino acids
Theoretical weight: 28.45 KDa
Source organism: Rhodococcus sp. NCIMB 9784
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93TU6 (Residues: 1-257; Coverage: 100%)
Gene name: camK
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand CAX 12 x CAX
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 83.28Å b: 132.008Å c: 135.424Å
α: 90° β: 94.11° γ: 90°
R-values:
R R work R free
0.165 0.164 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Emergent mechanistic diversity of enzyme-catalysed beta-diketone cleavage.
Grogan G. (2005)
2 other articles

4 mentions without citation

Strategies for carbohydrate recognition by the mannose 6-phosphate receptors.
Dahms et al. (2008)
3 more