› 1t0j

X-ray diffraction
2Å resolution

Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit

Released:
DOI: 10.2210/pdb1t0j/pdb
PDB entry 1t0j coloured by chain, front view.
PDB entry 1t0j coloured by chain, side view.
PDB entry 1t0j coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
Van Petegem F, Clark KA, Chatelain FC, Minor DL
Nature 429 671-5 (2004)
PMID: 15141227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171837 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Voltage-dependent L-type calcium channel subunit beta-2 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VGC3 (Residues: 68-196; Coverage: 20%)
Gene names: Cacnb2, Cacnlb2
Sequence domains: Voltage gated calcium channel subunit beta domain 4Aa N terminal
Structure domains: SH3 Domains
Voltage-dependent L-type calcium channel subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 224 amino acids
Theoretical weight: 25.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VGC3 (Residues: 254-476; Coverage: 34%)
Gene names: Cacnb2, Cacnlb2
Sequence domains: Guanylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Voltage-dependent L-type calcium channel subunit alpha-1C Chain: C
Molecule details ›
Chain: C
Length: 20 amino acids
Theoretical weight: 2.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13936 (Residues: 428-445; Coverage: 1%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 36.756Å b: 45.33Å c: 60.652Å
α: 96.81° β: 102.46° γ: 98.53°
R-values:
R R work R free
0.202 0.2 0.242
Expression system: Escherichia coli

Quick links

Citations

87 review citations

Voltage-gated calcium channels.
Catterall WA. (2011)
86 more

20 mentions without citation

Progress in the structural understanding of voltage-gated calcium channel (CaV) function and modulation.
Minor et al. (2010)
19 more