› 1t2f

X-ray diffraction
3Å resolution

Human B lactate dehydrogenase complexed with NAD+ and 4-hydroxy-1,2,5-oxadiazole-3-carboxylic acid

Released:
DOI: 10.2210/pdb1t2f/pdb
PDB entry 1t2f coloured by chain, front view.
PDB entry 1t2f coloured by chain, side view.
PDB entry 1t2f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and activity of a series of azole-based compounds with lactate dehydrogenase-directed anti-malarial activity.
Cameron A, Read J, Tranter R, Winter VJ, Sessions RB, Brady RL, Vivas L, Easton A, Kendrick H, Croft SL, Barros D, Lavandera JL, Martin JJ, Risco F, García-Ochoa S, Gamo FJ, Sanz L, Leon L, Ruiz JR, Gabarró R, Mallo A, Gómez de las Heras F
J Biol Chem 279 31429-39 (2004)
PMID: 15117937

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase B chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 333 amino acids
Theoretical weight: 36.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07195 (Residues: 2-334; Coverage: 100%)
Gene name: LDHB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand OXQ 4 x OXQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21212
Unit cell:
a: 137.397Å b: 160.443Å c: 59.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.215 0.321
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Opportunities and challenges in antiparasitic drug discovery.
Pink et al. (2005)
12 more

7 mentions without citation

Small-molecule inhibitors of human LDH5.
Granchi et al. (2013)
6 more