› 1t7p

X-ray diffraction
2.2Å resolution

T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN

Released:
DOI: 10.2210/pdb1t7p/pdb
PDB entry 1t7p coloured by chain, front view.
PDB entry 1t7p coloured by chain, side view.
PDB entry 1t7p coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution.
Doublié S, Tabor S, Long AM, Richardson CC, Ellenberger T
Nature 391 251-8 (1998)
PMID: 9440688

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114632 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 698 amino acids
Theoretical weight: 79.09 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00581 (Residues: 1-704; Coverage: 99%)
Gene name: 5
Sequence domains: DNA polymerase family A
Structure domains:
Thioredoxin 1 Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli O157:H7
Expression system: Not provided
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
DNA (5'-D(P*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*2DA)-3') Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.33 KDa
DNA (5'-D(P*CP*CP*TP*TP*GP*GP*CP*AP*CP*TP*GP*GP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 13 nucleotides
Theoretical weight: 3.94 KDa

Ligands and Environments

2 bound ligands:
Ligand DG3 1 x DG3
Ligand MG 3 x MG
1 modified residue:
Ligand 2DA 1 x 2DA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 106.6Å b: 216.3Å c: 52.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

118 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
117 more

53 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
52 more