› 1ta3

X-ray diffraction
1.7Å resolution

Crystal Structure of xylanase (GH10) in complex with inhibitor (XIP)

Released:
DOI: 10.2210/pdb1ta3/pdb
PDB entry 1ta3 coloured by chain, front view.
PDB entry 1ta3 coloured by chain, side view.
PDB entry 1ta3 coloured by chain, top view.
Source organisms:
Primary publication:
The dual nature of the wheat xylanase protein inhibitor XIP-I: structural basis for the inhibition of family 10 and family 11 xylanases.
Payan F, Leone P, Porciero S, Furniss C, Tahir T, Williamson G, Durand A, Manzanares P, Gilbert HJ, Juge N, Roussel A
J Biol Chem 279 36029-37 (2004)
PMID: 15181003

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169399 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Xylanase inhibitor protein 1 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.32 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: Q8L5C6 (Residues: 31-304; Coverage: 100%)
Gene name: XIPI
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases
Endo-1,4-beta-xylanase C Chain: B
Molecule details ›
Chain: B
Length: 303 amino acids
Theoretical weight: 33.06 KDa
Source organism: Aspergillus nidulans
Expression system: Aspergillus nidulans
UniProt:
  • Canonical: Q00177 (Residues: 25-327; Coverage: 100%)
Gene names: AN1818, xlnC
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 46.73Å b: 75.872Å c: 159.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.166
Expression system: Aspergillus nidulans

Quick links

Citations

11 review citations

Recent advances in plant cell wall proteomics.
Jamet et al. (2008)
10 more

7 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
6 more