› 1tee

X-ray diffraction
2.9Å resolution

Crystal structure of C205F mutant of PKS18 from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1tee/pdb
PDB entry 1tee coloured by chain, front view.
PDB entry 1tee coloured by chain, side view.
PDB entry 1tee coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites.
Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R
Nat Struct Mol Biol 11 894-900 (2004)
PMID: 15286723

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-pyrone synthesis polyketide synthase-like Pks18 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 42.12 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPF1 (Residues: 1-393; Coverage: 100%)
Gene names: Rv1372, pks18
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P1
Unit cell:
a: 59.952Å b: 80.431Å c: 99.975Å
α: 108.15° β: 93.14° γ: 103.88°
R-values:
R R work R free
0.221 0.221 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases.
Abe et al. (2010)
7 more

1 mention without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)