› 1tf6

X-ray diffraction
3.1Å resolution

CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RNA GENE INTERNAL CONTROL REGION

Released:
DOI: 10.2210/pdb1tf6/pdb
PDB entry 1tf6 coloured by chain, front view.
PDB entry 1tf6 coloured by chain, side view.
PDB entry 1tf6 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex.
Nolte RT, Conlin RM, Harrison SC, Brown RS
Proc Natl Acad Sci U S A 95 2938-43 (1998)
PMID: 9501194

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114643 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor IIIA Chains: A, D
Molecule details ›
Chains: A, D
Length: 190 amino acids
Theoretical weight: 22.13 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03001 (Residues: 23-212; Coverage: 52%)
Gene name: gtf3a
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*CP*GP*GP*GP*CP*CP*TP*GP*GP*TP*TP*AP*GP*TP*AP*C P*CP*TP*GP*GP*AP* TP*GP*GP*GP*AP*GP*AP*CP*C)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 31 nucleotides
Theoretical weight: 9.63 KDa
Source organism: Xenopus laevis
Expression system: Not provided
DNA (5'-D(*TP*GP*GP*TP*CP*TP*CP*CP*CP*AP*TP*CP*CP*AP*GP*GP*T P*AP*CP*TP*AP*AP* CP*CP*AP*GP*GP*CP*CP*CP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 31 nucleotides
Theoretical weight: 9.43 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P1
Unit cell:
a: 64.18Å b: 64.712Å c: 78.035Å
α: 90.07° β: 92.98° γ: 102.95°
R-values:
R R work R free
0.308 0.308 0.363
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

32 review citations

Intrinsically disordered protein.
Dunker et al. (2001)
31 more

10 mentions without citation

Macromolecular mimicry.
Nissen et al. (2000)
9 more