› 1tf9

X-ray diffraction
1.3Å resolution

Streptomyces griseus aminopeptidase complexed with P-Iodo-L-Phenylalanine

Released:
DOI: 10.2210/pdb1tf9/pdb
PDB entry 1tf9 coloured by chain, front view.
PDB entry 1tf9 coloured by chain, side view.
PDB entry 1tf9 coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Binding of inhibitory aromatic amino acids to Streptomyces griseus aminopeptidase.
Reiland V, Gilboa R, Spungin-Bialik A, Schomburg D, Shoham Y, Blumberg S, Shoham G
Acta Crystallogr D Biol Crystallogr 60 1738-46 (2004)
PMID: 15388919

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminopeptidase S Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 29.75 KDa
Source organism: Streptomyces griseus
UniProt:
  • Canonical: P80561 (Residues: 46-329; Coverage: 71%)
Gene name: SGR_5809
Sequence domains: Peptidase family M28
Structure domains: Zn peptidases

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 1 x CA
Ligand PHI 1 x PHI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P41212
Unit cell:
a: 61.053Å b: 61.053Å c: 144.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.122 0.172

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Citations

7 citation in other articles

Glu(332) in the Nicastrin ectodomain is essential for gamma-secretase complex maturation but not for its activity.
Chávez-Gutiérrez et al. (2008)
6 more