› 1tfw

X-ray diffraction
2.2Å resolution

How CCA is added to the 3' end of immature tRNA without the use of an oligonucleotide template

Released:
DOI: 10.2210/pdb1tfw/pdb
PDB entry 1tfw coloured by chain, front view.
PDB entry 1tfw coloured by chain, side view.
PDB entry 1tfw coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template.
Xiong Y, Steitz TA
Nature 430 640-5 (2004)
PMID: 15295590

Function and Biology Details

Reaction catalysed: 
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-219121 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct RNA molecules
Macromolecules (5 distinct):
CCA-adding enzyme Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 437 amino acids
Theoretical weight: 51.47 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
Structure domains:
5'-R(*GP*CP*GP*GP*AP*UP*CP*CP*GP*CP*AP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*GP*CP*GP*GP*AP*UP*CP*CP*GP*CP*AP*CP*C)-3' Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 13 nucleotides
Theoretical weight: 4.13 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*GP*CP*GP*GP*AP*CP*CP*CP*GP*CP*AP*C)-3' Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*CP*GP*CP*GP*GP*AP*UP*CP*CP*GP*CP*AP*C)-3' Chain: J
Molecule details ›
Chain: J
Length: 13 nucleotides
Theoretical weight: 4.13 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 116.641Å b: 84.77Å c: 135.813Å
α: 90° β: 104.66° γ: 90°
R-values:
R R work R free
0.195 0.192 0.242
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

24 review citations

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
23 more

5 mentions without citation

Determinants of substrate specificity in RNA-dependent nucleotidyl transferases.
Martin et al. (2008)
4 more