› 1tgh

X-ray diffraction
2.9Å resolution

TATA BINDING PROTEIN (TBP)/DNA COMPLEX

Released:
DOI: 10.2210/pdb1tgh/pdb
PDB entry 1tgh coloured by chain, front view.
PDB entry 1tgh coloured by chain, side view.
PDB entry 1tgh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
How proteins recognize the TATA box.
Juo ZS, Chiu TK, Leiberman PM, Baikalov I, Berk AJ, Dickerson RE
J Mol Biol 261 239-54 (1996)
PMID: 8757291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114644 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P20226 (Residues: 155-339; Coverage: 55%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (5'-D(*CP*GP*TP*AP*TP*AP*TP*AP*TP*AP*CP*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 66.965Å b: 67.397Å c: 86.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.294
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

28 review citations

The RNA polymerase II core promoter.
Smale et al. (2003)
27 more

14 mentions without citation

Inhibition of transcription by platinum antitumor compounds.
Todd et al. (2009)
13 more