› 1thj

X-ray diffraction
2.8Å resolution

CARBONIC ANHYDRASE FROM METHANOSARCINA

Released:
DOI: 10.2210/pdb1thj/pdb
PDB entry 1thj coloured by chain, front view.
PDB entry 1thj coloured by chain, side view.
PDB entry 1thj coloured by chain, top view.
Source organism: Methanosarcina thermophila
Primary publication:
A left-hand beta-helix revealed by the crystal structure of a carbonic anhydrase from the archaeon Methanosarcina thermophila.
Kisker C, Schindelin H, Alber BE, Ferry JG, Rees DC
EMBO J 15 2323-30 (1996)
PMID: 8665839

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-154203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 214 amino acids
Theoretical weight: 23.02 KDa
Source organism: Methanosarcina thermophila
Expression system: Not provided
UniProt:
  • Canonical: P40881 (Residues: 35-247; Coverage: 100%)
Gene name: MSTHT_0588
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P43212
Unit cell:
a: 71.84Å b: 71.84Å c: 333.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.295
Expression system: Not provided

Quick links

Citations

25 review citations

Carbonic anhydrases: current state of the art, therapeutic applications and future prospects.
Pastorekova et al. (2004)
24 more

6 mentions without citation

Biophysical highlights from 54 years of macromolecular crystallography.
Richardson et al. (2014)
5 more