› 1tiy

X-ray diffraction
2.5Å resolution

X-RAY STRUCTURE OF GUANINE DEAMINASE FROM BACILLUS SUBTILIS NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET SR160

Released:
DOI: 10.2210/pdb1tiy/pdb
PDB entry 1tiy coloured by chain, front view.
PDB entry 1tiy coloured by chain, side view.
PDB entry 1tiy coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kuzin AP, Vorobiev S, Edstrom W, Forouhar F, Acton T, Shastry R, Ma L-C, Chiang Y-W, Montelione G, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Guanine + H(2)O = xanthine + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.34 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34598 (Residues: 1-156; Coverage: 100%)
Gene names: BSU13170, gde, guaD
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 99.287Å b: 99.287Å c: 72.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.273
Expression system: Escherichia coli BL21(DE3)

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