› 1tjj

X-ray diffraction
2Å resolution

Human GM2 Activator Protein PAF complex

Released:
DOI: 10.2210/pdb1tjj/pdb
PDB entry 1tjj coloured by chain, front view.
PDB entry 1tjj coloured by chain, side view.
PDB entry 1tjj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evidence for lipid packaging in the crystal structure of the GM2-activator complex with platelet activating factor.
Wright CS, Mi LZ, Rastinejad F
J Mol Biol 342 585-92 (2004)
PMID: 15327957

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
homo trimer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ganglioside GM2 activator Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 164 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17900 (Residues: 32-193; Coverage: 95%)
Gene name: GM2A
Sequence domains: ML domain
Structure domains: Ganglioside GM2 activator

Ligands and Environments

7 bound ligands:
Ligand ACT 5 x ACT
Ligand CL 3 x CL
Ligand EPE 2 x EPE
Ligand PFS 3 x PFS
Ligand LPE 3 x LPE
Ligand IPA 5 x IPA
Ligand DAO 1 x DAO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 63.47Å b: 86.51Å c: 120.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Lysosomal degradation of membrane lipids.
Kolter et al. (2010)
3 more

3 mentions without citation

The role of hydrophobic interactions in positioning of peripheral proteins in membranes.
Lomize et al. (2007)
2 more