› 1tjl

X-ray diffraction
2Å resolution

Crystal structure of transcription factor DksA from E. coli

Released:
DOI: 10.2210/pdb1tjl/pdb
PDB entry 1tjl coloured by chain, front view.
PDB entry 1tjl coloured by chain, side view.
PDB entry 1tjl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Regulation through the secondary channel--structural framework for ppGpp-DksA synergism during transcription.
Perederina A, Svetlov V, Vassylyeva MN, Tahirov TH, Yokoyama S, Artsimovitch I, Vassylyev DG
Cell 118 297-309 (2004)
PMID: 15294156

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase-binding transcription factor DksA Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 151 amino acids
Theoretical weight: 17.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABS1 (Residues: 1-151; Coverage: 100%)
Gene names: JW0141, b0145, dksA
Sequence domains:
Structure domains: DksA, coiled-coil domain

Ligands and Environments

1 bound ligand:
Ligand ZN 10 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 91.319Å b: 96.593Å c: 117.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.228 0.268
Expression system: Escherichia coli

Quick links

Citations

54 review citations

(p)ppGpp: still magical?
Potrykus et al. (2008)
53 more

19 mentions without citation

Recent functional insights into the role of (p)ppGpp in bacterial physiology.
Hauryliuk et al. (2015)
18 more