Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Cytochrome f and Plastocyanin A, chloroplastic (preferred)
PDBe Complex ID:
PDB-CPX-132380 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plastocyanin A, chloroplastic
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 10.49 KDa
Source organism: Populus nigra
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Length: 99 amino acids
Theoretical weight: 10.49 KDa
Source organism: Populus nigra
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P00299 (Residues: 70-168; Coverage: 59%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome f
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 27.55 KDa
Source organism: Brassica rapa subsp. rapa
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
Sequence domains:
Structure domains:
Length: 252 amino acids
Theoretical weight: 27.55 KDa
Source organism: Brassica rapa subsp. rapa
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
- Canonical: P36438 (Residues: 36-287; Coverage: 88%)
Sequence domains:
Structure domains:
Ligands and Environments
Experiments and Validation Details
Refinement method:
Rigid body docking using intermolecular pseudocontact shifts restraints, chemical shift perturbation restraints and electrostatic restraints.
Expression systems:
- Escherichia coli BL21(DE3)
- Escherichia coli str. K-12 substr. W3110
