› 1tlf

X-ray diffraction
2.6Å resolution

UNPRECEDENTED QUATERNARY STRUCTURE OF E. COLI LAC REPRESSOR CORE TETRAMER: IMPLICATIONS FOR DNA LOOPING

Released:
DOI: 10.2210/pdb1tlf/pdb
PDB entry 1tlf coloured by chain, front view.
PDB entry 1tlf coloured by chain, side view.
PDB entry 1tlf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of lac repressor core tetramer and its implications for DNA looping.
Friedman AM, Fischmann TO, Steitz TA
Science 268 1721-7 (1995)
PMID: 7792597

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 32.15 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P03023 (Residues: 60-360; Coverage: 84%)
Gene names: JW0336, b0345, lacI
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand EMC 4 x EMC
Ligand IPT 4 x IPT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 90.16Å b: 64.73Å c: 117.94Å
α: 90° β: 91.75° γ: 90°
R-values:
R R work R free
0.222 0.222 not available
Expression system: Not provided

Quick links

Citations

26 review citations

Atomic structure and specificity of bacterial periplasmic receptors for active transport and chemotaxis: variation of common themes.
Quiocho et al. (1996)
25 more

12 mentions without citation

'Deadman' and 'Passcode' microbial kill switches for bacterial containment.
Chan et al. (2016)
11 more