› 1to9

X-ray diffraction
2.4Å resolution

Crystal structure of THI-4 protein from Bacillus subtilis

Released:
DOI: 10.2210/pdb1to9/pdb
PDB entry 1to9 coloured by chain, front view.
PDB entry 1to9 coloured by chain, side view.
PDB entry 1to9 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Rajan SS, Shuvalova L, Yang X, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-150335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aminopyrimidine aminohydrolase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 30.52 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25052 (Residues: 1-236; Coverage: 100%)
Gene names: BSU11650, tenA
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like
Aminopyrimidine aminohydrolase Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 30.55 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25052 (Residues: 1-236; Coverage: 100%)
Gene names: BSU11650, tenA
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

1 bound ligand:
Ligand HMH 2 x HMH
2 modified residues:
Ligand MSE 14 x MSE
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P41212
Unit cell:
a: 58.38Å b: 58.38Å c: 296.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.257
Expression system: Escherichia coli BL21(DE3)

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