› 1tps

X-ray diffraction
1.9Å resolution

ATOMIC STRUCTURE OF THE TRYPSIN-A90720A COMPLEX: A UNIFIED APPROACH TO STRUCTURE AND FUNCTION

Released:
DOI: 10.2210/pdb1tps/pdb
PDB entry 1tps coloured by chain, front view.
PDB entry 1tps coloured by chain, side view.
PDB entry 1tps coloured by chain, top view.
Source organisms:
Primary publication:
Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function.
Lee AY, Smitka TA, Bonjouklian R, Clardy J
Chem Biol 1 113-7 (1994)
PMID: 9383379

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133346 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
INHIBITOR A90720A Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.08 KDa
Source organism: Cyanobacteriota

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand DLE 1 x DLE

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 63.74Å b: 63.55Å c: 69.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 0.197
Expression system: Not provided

Quick links

Citations

7 review citations

Cyclic peptides and depsipeptides from cyanobacteria: a review.
Moore RE. (1996)
6 more

2 mentions without citation

Side-chain flexibility in protein-ligand binding: the minimal rotation hypothesis.
Zavodszky et al. (2005)
1 more