› 1tte

Solution NMR

The Structure of a Class II ubiquitin-conjugating enzyme, Ubc1.

Released:
DOI: 10.2210/pdb1tte/pdb
PDB entry 1tte coloured by chain, ensemble of 21 models, front view.
PDB entry 1tte coloured by chain, ensemble of 21 models, side view.
PDB entry 1tte coloured by chain, ensemble of 21 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Solution structure of the flexible class II ubiquitin-conjugating enzyme Ubc1 provides insights for polyubiquitin chain assembly.
Merkley N, Shaw GS
J Biol Chem 279 47139-47 (2004)
PMID: 15328341

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 1 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.19 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P21734 (Residues: 1-215; Coverage: 100%)
Gene names: UBC1, YD9395.10, YDR177W
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

6 review citations

Building ubiquitin chains: E2 enzymes at work.
Ye et al. (2009)
5 more

10 mentions without citation

Cellular strategies for making monoubiquitin signals.
Ramanathan et al. (2012)
9 more