› 1ttu

X-ray diffraction
2.85Å resolution

Crystal Structure of CSL bound to DNA

Released:
DOI: 10.2210/pdb1ttu/pdb
PDB entry 1ttu coloured by chain, front view.
PDB entry 1ttu coloured by chain, side view.
PDB entry 1ttu coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the nuclear effector of Notch signaling, CSL, bound to DNA.
Kovall RA, Hendrickson WA
EMBO J 23 3441-51 (2004)
PMID: 15297877

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114658 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Suppressor of hairless protein homolog Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 53.76 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: V6CLJ5 (Residues: 309-780; Coverage: 60%)
Gene names: K08B4.1, lag-1
Sequence domains:
Structure domains:
5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID, NSLS BEAMLINE X4A
Spacegroup: P61
Unit cell:
a: 127.547Å b: 127.547Å c: 97.604Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Notch signalling: a simple pathway becomes complex.
Bray SJ. (2006)
19 more

17 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
16 more