› 1txd

X-ray diffraction
2.13Å resolution

Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF

Released:
DOI: 10.2210/pdb1txd/pdb
PDB entry 1txd coloured by chain, front view.
PDB entry 1txd coloured by chain, side view.
PDB entry 1txd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor.
Kristelly R, Gao G, Tesmer JJ
J Biol Chem 279 47352-62 (2004)
PMID: 15331592

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 12 Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 44.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZN5 (Residues: 766-1138; Coverage: 24%)
Gene names: ARHGEF12, KIAA0382, LARG
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 193.606Å b: 45.859Å c: 74.73Å
α: 90° β: 107.49° γ: 90°
R-values:
R R work R free
0.237 0.235 0.274
Expression system: Escherichia coli

Quick links

Citations

16 review citations

GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors.
Rossman et al. (2005)
15 more

5 mentions without citation

Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors.
Aittaleb et al. (2010)
4 more