› 1txt

X-ray diffraction
2.5Å resolution

Staphylococcus aureus 3-hydroxy-3-methylglutaryl-CoA synthase

Released:
DOI: 10.2210/pdb1txt/pdb
PDB entry 1txt coloured by chain, front view.
PDB entry 1txt coloured by chain, side view.
PDB entry 1txt coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Staphylococcus aureus 3-hydroxy-3-methylglutaryl-CoA synthase: crystal structure and mechanism.
Campobasso N, Patel M, Wilding IE, Kallender H, Rosenberg M, Gwynn MN
J Biol Chem 279 44883-8 (2004)
PMID: 15292254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HMG-CoA synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 43.33 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FD87 (Residues: 1-388; Coverage: 100%)
Gene name: mvaS
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand CAA 4 x CAA
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 119.947Å b: 64.482Å c: 121.91Å
α: 90° β: 97.17° γ: 90°
R-values:
R R work R free
0.191 0.187 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
3 more

5 mentions without citation

3-hydroxy-3-methylglutaryl-CoA synthase intermediate complex observed in "real-time".
Theisen et al. (2004)
4 more