› 1tz9

X-ray diffraction
2.9Å resolution

Crystal Structure of the Putative Mannonate Dehydratase from Enterococcus faecalis, Northeast Structural Genomics Target EfR41

Released:
DOI: 10.2210/pdb1tz9/pdb
PDB entry 1tz9 coloured by chain, front view.
PDB entry 1tz9 coloured by chain, side view.
PDB entry 1tz9 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Forouhar F, Chen Y, Xiao R, Cooper B, Shastry R, Acton TA, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannonate dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 42.04 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82ZC9 (Residues: 1-357; Coverage: 100%)
Gene names: EF_3135, uxuA
Sequence domains: D-mannonate dehydratase (UxuA)
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 49.34Å b: 89.941Å c: 156.756Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.221 0.292
Expression system: Escherichia coli BL21(DE3)

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