› 1tzk

X-ray diffraction
2Å resolution

Crystal structure of 1-aminocyclopropane-1-carboxylate-deaminase complexed with alpha-keto-butyrate

Released:
DOI: 10.2210/pdb1tzk/pdb
PDB entry 1tzk coloured by chain, front view.
PDB entry 1tzk coloured by chain, side view.
PDB entry 1tzk coloured by chain, top view.
Source organism: Pseudomonas sp. ACP
Primary publication:
Structural analysis of Pseudomonas 1-aminocyclopropane-1-carboxylate deaminase complexes: insight into the mechanism of a unique pyridoxal-5'-phosphate dependent cyclopropane ring-opening reaction.
Karthikeyan S, Zhou Q, Zhao Z, Kao CL, Tao Z, Robinson H, Liu HW, Zhang H
Biochemistry 43 13328-39 (2004)
PMID: 15491139

Function and Biology Details

Reaction catalysed: 
(1a) 1-aminocyclopropane-1-carboxylate = 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 338 amino acids
Theoretical weight: 36.71 KDa
Source organism: Pseudomonas sp. ACP
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00740 (Residues: 1-338; Coverage: 100%)
Gene name: acdS
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand 2KT 2 x 2KT
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 67.664Å b: 68.446Å c: 350.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.218 0.255
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture.
Saleem et al. (2007)
5 more