› 1u0c

X-ray diffraction
2.5Å resolution

Y33C Mutant of Homing endonuclease I-CreI

Released:
DOI: 10.2210/pdb1u0c/pdb
PDB entry 1u0c coloured by chain, ensemble of 2 models, front view.
PDB entry 1u0c coloured by chain, ensemble of 2 models, side view.
PDB entry 1u0c coloured by chain, ensemble of 2 models, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Isolation and characterization of new homing endonuclease specificities at individual target site positions.
Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R, Stoddard BL, Seligman LM
J Mol Biol 342 31-41 (2004)
PMID: 15313605

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114667 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.66 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 1-163; Coverage: 100%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*TP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*AP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(*CP*GP*TP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*AP*GP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 43.089Å b: 68.021Å c: 87.182Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.23 0.214 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

The CRISPR tool kit for genome editing and beyond.
Adli M. (2018)
15 more

2 mentions without citation

phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine et al. (2010)
1 more