› 1u1b

X-ray diffraction
2Å resolution

Structure of bovine pancreatic Ribonuclease A in complex with 3'-phosphothymidine (3'-5')-pyrophosphate adenosine 3'-phosphate

Released:
DOI: 10.2210/pdb1u1b/pdb
PDB entry 1u1b coloured by chain, front view.
PDB entry 1u1b coloured by chain, side view.
PDB entry 1u1b coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Conservation of mus-ms enzyme motions in the apo- and substrate-mimicked state.
Beach H, Cole R, Gill ML, Loria JP
J Am Chem Soc 127 9167-76 (2005)
PMID: 15969595

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand PAX 2 x PAX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 31.086Å b: 75.861Å c: 51.821Å
α: 90° β: 106.31° γ: 90°
R-values:
R R work R free
0.218 0.218 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The role of dynamic conformational ensembles in biomolecular recognition.
Boehr et al. (2009)
11 more

12 mentions without citation

Evolutionarily conserved linkage between enzyme fold, flexibility, and catalysis.
Ramanathan et al. (2011)
11 more