› 1u1f

X-ray diffraction
2.3Å resolution

Structure of e. coli uridine phosphorylase complexed to 5-(m-(benzyloxy)benzyl)acyclouridine (BBAU)

Released:
DOI: 10.2210/pdb1u1f/pdb
PDB entry 1u1f coloured by chain, front view.
PDB entry 1u1f coloured by chain, side view.
PDB entry 1u1f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for inhibition of Escherichia coli uridine phosphorylase by 5-substituted acyclouridines.
Bu W, Settembre EC, el Kouni MH, Ealick SE
Acta Crystallogr D Biol Crystallogr 61 863-72 (2005)
PMID: 15983408

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 256 amino acids
Theoretical weight: 27.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P12758 (Residues: 2-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

3 bound ligands:
Ligand PO4 6 x PO4
Ligand K 3 x K
Ligand 183 6 x 183
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 91.557Å b: 125.813Å c: 141.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.228
Expression system: Escherichia coli

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Citations

9 citation in other articles

Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy.
Roosild et al. (2009)
8 more

1 mention without citation

Induction of Fc-Mediated Effector Functions Against a Stabilized Inner Domain of HIV-1 gp120 Designed to Selectively Harbor the A32 Epitope Region.
Visciano et al. (2019)