› 1u1s

X-ray diffraction
1.6Å resolution

Hfq protein from Pseudomonas aeruginosa. Low-salt crystals

Released:
DOI: 10.2210/pdb1u1s/pdb
PDB entry 1u1s coloured by chain, front view.
PDB entry 1u1s coloured by chain, side view.
PDB entry 1u1s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of Pseudomonas aeruginosa Hfq protein.
Nikulin A, Stolboushkina E, Perederina A, Vassilieva I, Blaesi U, Moll I, Kachalova G, Yokoyama S, Vassylyev D, Garber M, Nikonov S
Acta Crystallogr D Biol Crystallogr 61 141-6 (2005)
PMID: 15681864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 82 amino acids
Theoretical weight: 9.11 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUM0 (Residues: 1-82; Coverage: 100%)
Gene names: PA4944, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 61Å b: 73.26Å c: 106.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.313 0.223 0.25
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Hfq structure, function and ligand binding.
Brennan et al. (2007)
9 more

11 mentions without citation

Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity.
Djapgne et al. (2021)
10 more