› 1u2q

X-ray diffraction
2.5Å resolution

Crystal structure of Mycobacterium tuberculosis Low Molecular Weight Protein Tyrosine Phosphatase (MPtpA) at 2.5A resolution with glycerol in the active site

Released:
DOI: 10.2210/pdb1u2q/pdb
PDB entry 1u2q coloured by chain, front view.
PDB entry 1u2q coloured by chain, side view.
PDB entry 1u2q coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of low-molecular-weight protein tyrosine phosphatase from Mycobacterium tuberculosis at 1.9-A resolution.
Madhurantakam C, Rajakumara E, Mazumdar PA, Saha B, Mitra D, Wiker HG, Sankaranarayanan R, Das AK
J Bacteriol 187 2175-81 (2005)
PMID: 15743966

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Low molecular weight protein-tyrosine phosphatase A Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 17.92 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIA1 (Residues: 1-163; Coverage: 100%)
Gene names: MTCY427.15, Rv2234, mptpA, ptpA
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 49.311Å b: 53.003Å c: 64.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.273
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure/function studies of Ser/Thr and Tyr protein phosphorylation in Mycobacterium tuberculosis.
Greenstein et al. (2005)
5 more

5 mentions without citation

Therapeutic Targeting of Protein Tyrosine Phosphatases from Mycobacterium tuberculosis.
Ruddraraju et al. (2020)
4 more