› 1u3f

X-ray diffraction
2.5Å resolution

Structural and Functional Characterization of a 5,10-Methenyltetrahydrofolate Synthetase from Mycoplasma pneumoniae (GI: 13508087)

Released:
DOI: 10.2210/pdb1u3f/pdb
PDB entry 1u3f coloured by chain, front view.
PDB entry 1u3f coloured by chain, side view.
PDB entry 1u3f coloured by chain, top view.
Source organism: Mycoplasma pneumoniae
Primary publication:
Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution.
Chen S, Shin DH, Pufan R, Kim R, Kim SH
Proteins 56 839-43 (2004)
PMID: 15281135

Function and Biology Details

Reaction catalysed: 
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formyltetrahydrofolate cyclo-ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 22.27 KDa
Source organism: Mycoplasma pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P75430 (Residues: 1-164; Coverage: 100%)
Gene names: H91_orf164, MP488, MPN_348
Sequence domains: 5-formyltetrahydrofolate cyclo-ligase family
Structure domains: NagB/RpiA/CoA transferase-like

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 45.135Å b: 74.369Å c: 110.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.268
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Diversification of catalytic activities and ligand interactions in the protein fold shared by the sugar isomerases, eIF2B, DeoR transcription factors, acyl-CoA transferases and methenyltetrahydrofolate synthetase.
Anantharaman et al. (2006)
6 more

1 mention without citation

Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489).
Meier et al. (2007)