› 1u46

X-ray diffraction
2Å resolution

Crystal Structure of the Unphosphorylated Kinase Domain of the Tyrosine Kinase ACK1

Released:
DOI: 10.2210/pdb1u46/pdb
PDB entry 1u46 coloured by chain, front view.
PDB entry 1u46 coloured by chain, side view.
PDB entry 1u46 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the phosphorylated and unphosphorylated kinase domains of the Cdc42-associated tyrosine kinase ACK1.
Lougheed JC, Chen RH, Mak P, Stout TJ
J Biol Chem 279 44039-45 (2004)
PMID: 15308621

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Activated CDC42 kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 33.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07912 (Residues: 109-395; Coverage: 28%)
Gene names: ACK1, TNK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 81.176Å b: 43.401Å c: 84.452Å
α: 90° β: 111.82° γ: 90°
R-values:
R R work R free
0.202 0.199 0.249
Expression system: Spodoptera frugiperda

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Citations

6 review citations

Mechanisms of RET signaling in cancer: current and future implications for targeted therapy.
Plaza-Menacho et al. (2014)
5 more

5 mentions without citation

Domain Architecture of the Nonreceptor Tyrosine Kinase Ack1.
Kan et al. (2023)
4 more