› 1u54

X-ray diffraction
2.8Å resolution

Crystal Structures of the Phosphorylated and Unphosphorylated Kinase Domains of the CDC42-associated Tyrosine Kinase ACK1 bound to AMP-PCP

Released:
DOI: 10.2210/pdb1u54/pdb
PDB entry 1u54 coloured by chain, front view.
PDB entry 1u54 coloured by chain, side view.
PDB entry 1u54 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the phosphorylated and unphosphorylated kinase domains of the Cdc42-associated tyrosine kinase ACK1.
Lougheed JC, Chen RH, Mak P, Stout TJ
J Biol Chem 279 44039-45 (2004)
PMID: 15308621

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170563 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Activated CDC42 kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07912 (Residues: 109-395; Coverage: 28%)
Gene names: ACK1, TNK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Activated CDC42 kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 33.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07912 (Residues: 109-395; Coverage: 28%)
Gene names: ACK1, TNK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ACP 2 x ACP
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 200.384Å b: 42.332Å c: 70.848Å
α: 90° β: 96.28° γ: 90°
R-values:
R R work R free
0.226 0.222 0.322
Expression system: Spodoptera frugiperda

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Citations

6 review citations

Shepherding AKT and androgen receptor by Ack1 tyrosine kinase.
Mahajan et al. (2010)
5 more

11 mentions without citation

Cell-free translation of integral membrane proteins into unilamelar liposomes.
Goren et al. (2009)
10 more