› 1u59

X-ray diffraction
2.3Å resolution

Crystal Structure of the ZAP-70 Kinase Domain in Complex with Staurosporine

Released:
DOI: 10.2210/pdb1u59/pdb
PDB entry 1u59 coloured by chain, front view.
PDB entry 1u59 coloured by chain, side view.
PDB entry 1u59 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The three-dimensional structure of the ZAP-70 kinase domain in complex with staurosporine: implications for the design of selective inhibitors.
Jin L, Pluskey S, Petrella EC, Cantin SM, Gorga JC, Rynkiewicz MJ, Pandey P, Strickler JE, Babine RE, Weaver DT, Seidl KJ
J Biol Chem 279 42818-25 (2004)
PMID: 15292186

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43403 (Residues: 327-606; Coverage: 45%)
Gene names: SRK, ZAP70
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 1 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 43.056Å b: 57.064Å c: 125.133Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.271
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

The Src, Syk, and Tec family kinases: distinct types of molecular switches.
Bradshaw JM. (2010)
8 more

18 mentions without citation

ZAP-70: an essential kinase in T-cell signaling.
Wang et al. (2010)
17 more