› 1u5h

X-ray diffraction
1.65Å resolution

Structure of Citrate Lyase beta subunit from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1u5h/pdb
PDB entry 1u5h coloured by chain, front view.
PDB entry 1u5h coloured by chain, side view.
PDB entry 1u5h coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structure and computational analysis of Mycobacterium tuberculosis protein CitE suggest a novel enzymatic function.
Goulding CW, Bowers PM, Segelke B, Lekin T, Kim CY, Terwilliger TC, Eisenberg D
J Mol Biol 365 275-83 (2007)
PMID: 17064730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate lyase subunit beta-like protein Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 28.58 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P9WPE1 (Residues: 1-273; Coverage: 100%)
Gene names: Rv2498c, citE
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: R32
Unit cell:
a: 91.214Å b: 91.214Å c: 221.233Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.268 0.268 0.272
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Metabolomic strategies for the identification of new enzyme functions and metabolic pathways.
Prosser et al. (2014)
2 more

6 mentions without citation

CLYBL is a polymorphic human enzyme with malate synthase and β-methylmalate synthase activity.
Strittmatter et al. (2014)
5 more