› 1u61

X-ray diffraction
2.15Å resolution

Crystal Structure of Putative Ribonuclease III from Bacillus cereus

Released:
DOI: 10.2210/pdb1u61/pdb
PDB entry 1u61 coloured by chain, front view.
PDB entry 1u61 coloured by chain, side view.
PDB entry 1u61 coloured by chain, top view.
Source organism: Bacillus cereus
Entry authors: Osipiuk J, Quartey P, Moy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mini-ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.68 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81J58 (Residues: 1-135; Coverage: 100%)
Gene names: BC_0111, mrnC
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease III domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4132
Unit cell:
a: 168.978Å b: 168.978Å c: 168.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.216
Expression system: Escherichia coli BL21(DE3)

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