› 1u6p

Solution NMR

NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein

Released:
DOI: 10.2210/pdb1u6p/pdb
PDB entry 1u6p coloured by chain, ensemble of 20 models, front view.
PDB entry 1u6p coloured by chain, ensemble of 20 models, side view.
PDB entry 1u6p coloured by chain, ensemble of 20 models, top view.
Source organism: Moloney murine leukemia virus
Primary publication:
Structural basis for packaging the dimeric genome of Moloney murine leukaemia virus.
D'Souza V, Summers MF
Nature 431 586-90 (2004)
PMID: 15457265

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136802 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleocapsid protein p10-Gag Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.38 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03332 (Residues: 479-534; Coverage: 10%)
Gene name: gag
Sequence domains: Zinc knuckle
Structure domains: Zinc finger, CCHC-type
101-MER Chain: B
Molecule details ›
Chain: B
Length: 101 nucleotides
Theoretical weight: 32.7 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided
Sequence domains: Gammaretrovirus core encapsidation signal

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand AP7 1 x AP7

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS, SIMULATED ANNEALING
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

32 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
31 more

4 mentions without citation

Sequence-specific binding of single-stranded RNA: is there a code for recognition?
Auweter et al. (2006)
3 more