› 1u6s

X-ray diffraction
2.3Å resolution

Crystal Structure of the Complex Between Mycobacterium Tuberculosis Beta-Ketoacyl-Acyl Carrier Protein Synthase III and Lauroyl Coenzyme A

Released:
DOI: 10.2210/pdb1u6s/pdb
PDB entry 1u6s coloured by chain, front view.
PDB entry 1u6s coloured by chain, side view.
PDB entry 1u6s coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A.
Musayev F, Sachdeva S, Scarsdale JN, Reynolds KA, Wright HT
J Mol Biol 346 1313-21 (2005)
PMID: 15713483

Function and Biology Details

Reaction catalysed: 
Dodecanoyl-CoA + a malonyl-[acyl-carrier protein] = a 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 34.87 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNG3 (Residues: 1-335; Coverage: 100%)
Gene names: MTCY25D10.12c, Rv0533c, fabH
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand DCC 2 x DCC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 55.57Å b: 63.19Å c: 55.33Å
α: 113.25° β: 100.82° γ: 92.66°
R-values:
R R work R free
0.208 0.206 0.247
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Membrane lipid homeostasis in bacteria.
Zhang et al. (2008)
5 more

5 mentions without citation

Discordant and chameleon sequences: their distribution and implications for amyloidogenicity.
Gendoo et al. (2011)
4 more