› 1u7x

X-ray diffraction
3Å resolution

crystal structure of a mutant M. jannashii tyrosyl-tRNA synthetase specific for O-methyl-tyrosine

Released:
DOI: 10.2210/pdb1u7x/pdb
PDB entry 1u7x coloured by chain, front view.
PDB entry 1u7x coloured by chain, side view.
PDB entry 1u7x coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.
Zhang Y, Wang L, Schultz PG, Wilson IA
Protein Sci 14 1340-9 (2005)
PMID: 15840835

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.81 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 45.371Å b: 185Å c: 93.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.229 0.308
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Expanding the genetic code.
Wang et al. (2006)
9 more

2 mentions without citation

Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.
Zhang et al. (2005)
1 more