› 1u83

X-ray diffraction
2.2Å resolution

PSL synthase from Bacillus subtilis

Released:
DOI: 10.2210/pdb1u83/pdb
PDB entry 1u83 coloured by chain, front view.
PDB entry 1u83 coloured by chain, side view.
PDB entry 1u83 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Cuff ME, Xu X, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + hydrogen sulfite
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphosulfolactate synthase Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O06739 (Residues: 1-252; Coverage: 100%)
Gene names: BSU10950, yitD
Sequence domains: (2R)-phospho-3-sulfolactate synthase (ComA)
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P213
Unit cell:
a: 92.071Å b: 92.071Å c: 92.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.219
Expression system: Escherichia coli BL21

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