› 1uas

X-ray diffraction
1.5Å resolution

Crystal structure of rice alpha-galactosidase

Released:
DOI: 10.2210/pdb1uas/pdb
PDB entry 1uas coloured by chain, front view.
PDB entry 1uas coloured by chain, side view.
PDB entry 1uas coloured by chain, top view.
Source organism: Oryza sativa
Primary publication:
Crystal structure of rice alpha-galactosidase complexed with D-galactose.
Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H
J Biol Chem 278 20313-8 (2003)
PMID: 12657636

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 39.99 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FXT4 (Residues: 56-417; Coverage: 100%)
Gene names: LOC_Os10g35110, OSJNBa0041P03, Os10g0493600
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLA 1 x GLA
Ligand SO4 1 x SO4
Ligand PT 1 x PT
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 63.656Å b: 71.42Å c: 86.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.178
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure and function of carbohydrate-binding module families 13 and 42 of glycoside hydrolases, comprising a β-trefoil fold.
Fujimoto Z. (2013)
2 more

17 mentions without citation

Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora.
Gloster et al. (2008)
16 more