› 1uek

X-ray diffraction
1.7Å resolution

Crystal structure of 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol kinase

Released:
DOI: 10.2210/pdb1uek/pdb
PDB entry 1uek coloured by chain, front view.
PDB entry 1uek coloured by chain, side view.
PDB entry 1uek coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis.
Wada T, Kuzuyama T, Satoh S, Kuramitsu S, Yokoyama S, Unzai S, Tame JR, Park SY
J Biol Chem 278 30022-7 (2003)
PMID: 12771135

Function and Biology Details

Reaction catalysed: 
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 29.29 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P83700 (Residues: 1-275; Coverage: 100%)
Gene names: TTHA0170, ispE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 48.603Å b: 66.21Å c: 76.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.218
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Molecular recognition in chemical and biological systems.
Persch et al. (2015)
8 more

2 mentions without citation

New Insight into Isoprenoids Biosynthesis Process and Future Prospects for Drug Designing in Plasmodium.
Saggu et al. (2016)
1 more