› 1uis

X-ray diffraction
2Å resolution

The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor

Released:
DOI: 10.2210/pdb1uis/pdb
PDB entry 1uis coloured by chain, front view.
PDB entry 1uis coloured by chain, side view.
PDB entry 1uis coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor.
Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J
J Biol Chem 278 44626-31 (2003)
PMID: 12909624

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP611 Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26.31 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ISF8 (Residues: 1-231; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ACY 2 x ACY
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6522
Unit cell:
a: 77.935Å b: 77.935Å c: 327.472Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.253
Expression system: Escherichia coli

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Citations

22 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
21 more

8 mentions without citation

Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes.
Stepanenko et al. (2008)
7 more