› 1ujw

X-ray diffraction
2.75Å resolution

Structure of the complex between BtuB and Colicin E3 Receptor binding domain

Released:
DOI: 10.2210/pdb1ujw/pdb
PDB entry 1ujw coloured by chain, front view.
PDB entry 1ujw coloured by chain, side view.
PDB entry 1ujw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of BtuB with bound colicin E3 R-domain implies a translocon.
Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA
Nat Struct Biol 10 948-54 (2003)
PMID: 14528295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132797 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:
Colicin E3 Chain: B
Molecule details ›
Chain: B
Length: 135 amino acids
Theoretical weight: 15.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00646 (Residues: 314-448; Coverage: 25%)
Gene names: ceaC, col
Sequence domains: Coiled-coil receptor-binding R-domain of colicin E2
Structure domains: Helix Hairpins

Ligands and Environments

5 bound ligands:
Ligand GP1 2 x GP1
Ligand LIM 2 x LIM
Ligand LDA 7 x LDA
Ligand AAE 1 x AAE
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 76.933Å b: 80.098Å c: 233.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.293
Expression system: Escherichia coli

Quick links

Citations

17 review citations

TonB or not TonB: is that the question?
Krewulak et al. (2011)
16 more

11 mentions without citation

Colicin biology.
Cascales et al. (2007)
10 more