› 1ukc

X-ray diffraction
2.1Å resolution

Crystal Structure of Aspergillus niger EstA

Released:
DOI: 10.2210/pdb1ukc/pdb
PDB entry 1ukc coloured by chain, front view.
PDB entry 1ukc coloured by chain, side view.
PDB entry 1ukc coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Aspergillus niger protein EstA defines a new class of fungal esterases within the alpha/beta hydrolase fold superfamily of proteins.
Bourne Y, Hasper AA, Chahinian H, Juin M, De Graaff LH, Marchot P
Structure 12 677-87 (2004)
PMID: 15062090

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Carboxylic ester hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 522 amino acids
Theoretical weight: 56.93 KDa
Source organism: Aspergillus niger
Expression system: Aspergillus niger
UniProt:
  • Canonical: Q6ED33 (Residues: 17-538; Coverage: 100%)
Gene names: CAN33_0010660, estA
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 10 x NAG
Ligand MAN 5 x MAN
Ligand SO4 2 x SO4
Ligand CL 1 x CL
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P63
Unit cell:
a: 167.489Å b: 167.489Å c: 112.827Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.158 0.184
Expression system: Aspergillus niger

Quick links

Citations

3 review citations

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei).
Stricker et al. (2008)
2 more

3 mentions without citation

Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Vici et al. (2015)
2 more