› 1ulu

X-ray diffraction
2Å resolution

Crystal structure of tt0143 from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1ulu/pdb
PDB entry 1ulu coloured by chain, front view.
PDB entry 1ulu coloured by chain, side view.
PDB entry 1ulu coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Ago H, Hamada K, Ida K, Kanda H, Sugahara M, Yamamoto M, Nodake Y, Kuramitsu S, Yokoyama S, Miyano M, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178169 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 261 amino acids
Theoretical weight: 28.47 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLI9 (Residues: 1-261; Coverage: 100%)
Gene name: TTHA0304
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 97.033Å b: 96.706Å c: 107.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.249
Expression system: Escherichia coli

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